Sunday, January 26, 2020

Analysis of Biochemical Compounds in ‘gynura Procumbens’

Analysis of Biochemical Compounds in ‘gynura Procumbens’ Extraction of Biochemical Compounds in ‘gynura Procumbens’ Using 3 Mixture Design and Gcms Name : Syazliana Syamira Binti Hashim NOVEMBER 2014 Gynura procumbens or known locally in Malaysia as ‘Sambung Nyawa’ or in chinese, it is called ‘Nan Hui Ye’ is a plant that was found in South East Asia usually inThailand, Indonesia and Malaysia.This plant belongs to the Asteraceae Family.It is a famous traditional herb in South East Asia for the treatment of diabetic, high cholesterol level, cancer, high blood pressure, kidney discomfort and inflammation.The present study aims to study the antidiabetic activity of Gynura procumbens leaves to manage diabetes and to study the potential toxicity for many of the biochemical compounds in this plant leaves by using the three mixture design and analysis by CGMS technique. In a mixture experiment, the independent factors are proportions of different components of a blend. The fact that the properties of the different factors must sum to 100% complicates the design as well as the analysis of mixture experiments.In this work,a mixture design will be applied to optimize solvent mixtures for selective extraction of biochemical compounds from Gynura procumbens leaves.Pressurized solvent extractions(PSE) will be performed at 313K and 20MPa,using homogeneous,ethanol(EtOH) and O solvent mixtures.The operating conditions to extract biochemical compounds from Gynura procumbens leaves will be optimized.The ranges of extraction temperatures and pressures are 25 60 and 8 – 25MPa, respectively.GC coupled with MS detection will be used to characterize the extracts. Introduction The modern lifestyle has become a major threat to the health of people around the world.There are many cases that involve arteriosclerosis, obesity, diabetes mellitus and cancers and these diseases remind us about our unhealthy lifestyles and thus give rise in circulatory system diseases such as hypertension, pre-hypertension and heart disease in developed countries (Johnson and Turner, 2005; Kearney et al., 2005). From the previous time,plants have often been used to cure human diseases (Stepp and Moerman,2001; Yesilada, 2005).Today,with the arising threat of intractable diseases,a research is to focus on the finding bioactive molecules from plants(Amos et al.,2003; Fatehi et al.,2005).The one such plant is ‘Sambung Nyawa’ or its scientific name is Gynura procumbens is a member of the Asteraceae family(Wiart,2002). Gynura procumbens is commonly used as a traditional medicinal plant in Malaysia to treat many diseases. Recently, the use of herbal medicines for complementary treatments of some diseases has been popular and researchers have shown that these herbs are commonly used by cancer patients to manage their diseases (Riboli and Norat, 2003; Van Duijnhoven et al., 2009). Pharmacologic studies also have proved that Gynura procumbens possesses anti-herpes simplex virus, (Nawawi et al.,1999), anti-ulcerogenic activities (Mahmood et al., 2010) anti-inflammatory (Iskander et al.,2002), and anticancer properties(Agustina et al.,2006). Problem Statement : Gynura procumbens had been given attention in the pharmacology of antidiabetic medicinal plants probably because of its solid evidences and efficiency in the traditional management of diabetes mellitus.But in contrast,the scientific reports on the antidiabetic activity of this plant had their own conflicts and inconsistent.For example,Zhang and Tan had reported that 95% ethanol extract improved glucose tolerance in STZ-induced diabetic rats, but not in normal rats(Zhang and Tan).These authors also reported that its aqueous extract to exert significant anti hyperglycemic action in STZ-induced diabetic rats.But after that,Akowuah et al., on the contrary indicated its glucose lowering effect in normal rats(Akowuah et al.,2001).In the most recent study, the extract of Gynura procumbens was reported to generate significant elevation in the fasting blood glucose(FBG) levels of normal rats but a decrease in diabetic rats(Hassan et al.,2010). Eventhough Gynura procumbens is useful in the treatment of many ailments,however there is a little toxicological information that is available regarding to the safety due to the repeated exposure.Thus,a study need to be conducted to improve these reports and give more useful informations about its widespread uses.Therefore,the present research is to study the antidiabetic activity of Gynura procumbens leaves to manage diabetes and also to study the potential toxicity for many of the biochemical compounds in Gynura procumbens leaves. Objectives : To study the antidiabetic activity of Gynura procumbens leaves to manage diabetes. To study the potential toxicity for many of the biochemical compounds in Gynura procumbens leaves. 2.0 Literature Review 2.1 General Overview of Gynura procumbens Gynura procumbens that is well-known in South East Asia has traditionally been used to cure rash, eruptive fevers, kidney disease, constipation, migraines, diabetes mellitus, hypertension and cancer(Perry,1980).Recently, pharmacological studies have state that this plant has anti-hyperglycaemic(Akowuah et al.,2001; Akowuah et al.,2002),anti-hyperlipidaemic(Zhang and Tan,2000),anti-inflammatory(Iskander et al.,2002) and blood hypertension reduction capabilities(Lam et al.,1998; Kim et al.,2006). The advantages of the traditional use of Gynura procumbens leaves have been supported the isolation and identification of several possible active chemical constituents from this herb, including flavonoids, tannins, saponins and terpenoids(Akowuah et al.,2002).Gynura procumbens is usually used as a traditional medicinal plant in Malaysia to cure many ailments. It is an annual ever- green shrub with a fleshy stem and purple tint.In Malaysia,this plant is called as ‘Sambung nyawa’, and it is commonly used in South-East Asia,especially in Malaysia, Indonesia, and Thailand. In spite of the various uses over long time periods, little toxicological information is available regarding safety following repeated exposure to Gynura procumbens. Currently, Malaysian authorities are paying more attention to the safety and potential toxicity of botanicals, including medical plants and edible materials. Therefore,the objective of the present study was to provide scientific data on the safety of Gynura procumbens, that focus on the acute and 90days (13weeks) sub-chronic toxicity of a methanol extract that was orally administered to Sprague Dawley (SD) rats. Gynura procumbens has recently received particular attention in the pharmacology of antidiabetic medicinal plants,probably because of its admitted empirical evidence and efficiency in the traditional management of diabetes mellitus.Besides that, these study designs are not targeted at natural product discovery or production of standardized herbal forms. Satisfactory research on medicinal plants beyond screening for biological activity should be conducted with the objective to systematically standardize and possess them into natural products or dosage forms which should effectively complement or supplement existing conventional measures(Ali et al.,2012). 2.2 Current Research of Gynura procumbens There is currently a good deal of research interest to utilize plant compounds against human diseases, that includes hypertension. The present study investigated the effects of different extracts and fractions from leaves of Gynura procumbens on rat atrial contraction in vitro. Isolated left and right atria were mounted in a 20-ml organ bath and they were allowed to equilibrate for 15 minutes before the application of the extracts or fractions. The extracts ( methanol extract (ME) and petroleum-ether extract (PE) ) and the fractions (chloroform fraction(CHL), ethyl-acetate fraction (EA), n-butanol fraction (NB) and water fraction (WA) of the methanol extract) were tested at three concentrations (0.25, 0.5 and 1.0 mg/ml), with a b-adrenergic agonist (isoprenaline) as a control. All the data on the contraction responses were log-transformed and had been analyzed. When exposed to the different extracts, both atria tended to exhibit greater contractive responses with the NB whereas cardiac contractions had a tendency to be reduced with most other extracts. For a given extract, the contraction responses were particularly greater at 0.5 mg/ml for the right atrium and at 1 mg/ml for the left atrium. For the further analysis is to focus on the NB fraction revealed that positive inotropism was greater in left atria exposed to highly-concentrated F2 and F3 sub-fractions. Taken together,the results suggest that NB extracts and fractions from the Gynura procumbens-leaf methanol extract have positive inotropic activities and, hence, can be considered as an alternative or traditional medicine against increased blood pressure in humans or can be used in strategies aimed at finding antihypertensive biomolecules from an accessible source. In each of these studies, the approach was not bioassay directed, an approach basis to the discovery of novel natural products from their natural sources and or to increase their standardization preparation to formulate into dosage forms for human usage after clinical trials. In summary, there is little analysis on the importance of the different extracts and fractions of Gynura procumbens on heart tissues. Therefore, the present study examined the effects of Gynura procumbens leaves extracts and fractions of the active extract on the contraction of left and right atria (LA and RA, respectively) isolated from Sprague Dawley (SD) rats with the aim of exploring the possibilities of its standardization or industrial scaling up for natural products against hypertension. 2.3 Functions of Gynura procumbens In South-East Asia, this plant is widely distributed and has often been used to treat diseases. In Indonesia, leaves of the Compositae family are routinely used for treatment in kidney diseases, eruptive fevers, rash, hypertension, diabetes mellitus, and hyperlipidemia(Perry,1980). In Thailand, where this plant is empirically used against topical inflammation, rheumatism, and viral ailments, Iskander and colleagues have reported the anti-inflammatory actions of its extracts(Iskander et al.,2002). Recently, pharmacological investigations in Singapore have shown that extracts of Gynura procumbens reduced serum cholesterol and triglyceride levels in streptozotocin induced diabetic rats(Zhang and Tan,2000). In Malaysia, evidence exists that Gynura procumbens has antidiabetic properties(Akowuah et al.,2001; Bohari et al.,2006). In this country, the currency of hypertension is high, but the levels of awareness, control, and treatment are still low(Rampal et al.,2008).This species has been used as a traditional medicine to treat inflammation, cancer, rheumatism, and viral infections. Among the various ethnobotanical practices of this plant, the application as a poultice for diverse skin diseases was found to be a universal usage in many countries (Perry, 1980). The leaves of this plant are not toxic(Rosidah et al., 2009). Gynura procumbensfrom Asteraceae family has been used as a traditional remedy for various skin diseases in certain areas of Southeast Asia(Junoh et al.,2011). 2.4 Related Diseases There were many attempts to control increased blood pressure that have been used as strategies to control arterial hypertension(Collins et al.,1990; Lang et al.,2001). Eventhough the treatment of arterial hypertension has been shown to be quite efficacious in reducing cardiovascular mortality and morbidity (Collins et al.,1990), hypertension control at the population level has been generally considered as not enough yet. In the United Kingdom, for instance, 94% of hypertensive patients still have their blood pressure higher than the normal(Colhoun et al.,1998), whereas in the United States, there is only 27% had a normal blood pressure following treatment(Burt et al.,1995). This intractability of hypertension has not changed, and recently there is a noticeable increase in hypertension cases(Kearney et al.,2004); about 26% of adults worldwide in 972 million are known to have hypertension(Kearney et al.,2005). Current optimism based on pharmacological therapy, which consists of developing drug formulations, is severely impeded by drug interactions, dose dependence, and adverse effects such as the possibility of depression. All in all,it is clear that the development of alternative strategies complementary to existing control methods is needed. 2.5 Acute and Sub-chronic Toxicity The Gynura procumbens methanol extract was suspended in 1% (w/v) carboxylmethylcellulose (CMC) and it was administered orally (1250, 2500, and 5000 mg/kg) in single doses to both female and males rats (n = 10; 5 males and 5 females). The rats need to fast overnight due to dosing. The general behaviour of the rat was continuously monitored for 1 hour after dosing, periodically during the first 24 hour (with special attention given during the first 4 hour), and daily thereafter for a total of 14 days. All animals were humanely killed by inhalation after 14 days.The selected vital organs were excised, weighed, and macroscopically examined(Ghosh,1984). Healthy rats of both sexes were randomly assigned to control and treatment groups (n = 10; 5 males and 5 females). The Gynura procumbens methanol extract was suspended in 1% CMC and administered orally on a daily basis for 90 days (13 weeks) at doses of 125, 250, or 500mg/kg. Based on the report by Suharmiati (2003), traditional healers typically prescribe seven leaves each day for an adult, which is equivalent to a 125 mg/day dose. Therefore, doses ranging from of 125–500mg/(kg day) were selected as the tested doses. All rats were anesthetized under inhalation at the end of 13 weeks. Blood samples were collected via cardiac puncture into non-heparinized and EDTA containing tubes for biochemical and haematological analyses, respectively (Winarto, 2003; Petterino and Argentino-Storino, 2006). After cardiac puncture, the rats were killed by clavicle dislocation.Those vital organs were excised, weighed, and macroscopically examined and then they were fixed in 10% formalin for hi stopathological study. 2.6 Diabetes Mellitus Diabetes causes oxidative stress through the autooxidation of glucose, protein glycation and lipid peroxidation; and as a consequence, structural and functional alterations occur in the lungs of diabetics, as well as other organs like the heart, brain, eyes and kidneys. There is growing, well-established evidence regarding such changes in diabetic lungs(Gulay et al.,2014). Oxidative stress plays an important role in the pathogenesis of diabetes mellitus, contributing not only to the development, but also to the progression of diabetes and its related complications. The immunosuppressive and antioxidant effects of resveratrol in attenuating the increased oxidative stress due to responses of ÃŽ ²-cells to leukocyte activation have been implicated in the prevention of type 1 diabetes mellitus. Resveratrol affords advantageous effects by decreasing the oxidative injury and the recruitment of the nutritive precapillary arterioles in the context of disease states associated with insulin re sistance, such as metabolic syndrome, pre-diabetes, and type 2 diabetes mellitus. Globally, more than 366 million individuals currently live with diabetes mellitus, with this figure expected to rise to 552 million by 2030 (Whiting et al., 2011). The World Health Organization estimates that around 1.1 million deaths occur per year as a result of the disease (World Health Organization, 2011). There are around 2.9 million individuals in England currently have diagnosed with diabetes, a currency of 4.5% (NHS The Information Centre, 2011). Projections for England estimate that by 2030 over 4.5 million people will be living with diabetes (APHO, 2011). The number of individuals that are living with diabetes means that if there was found to be an excess in mortality caused by the disease, this could result in a large number of premature deaths. The relation between diabetes and increased all-cause death, particularly from cardiovascular disease (CVD) and renal disease, is well recognized. C There is a limited amount of evidence relating to diabetes and respiratory disease mortality (Dawson et al., 2008;De Marco et al., 1999andMurugan and Sharma, 2008).Current evidence suggests a biological link between inflammation, reduced levels of adiponectin within the body and the development of diabetes and respiratory disease (Sevenoaks Stockley, 2006). De Marco et al. concluded that these may be caused by differences in the severity of diabetes or differing in national treatment cultures (De Marco et al., 1999). Therefore, research is required that further explores the relation between diabetes and mortality from cancer and respiratory disease. Current research indicates that, for mortality from a number of causes, it may be diabetes-related comorbidities that increase an individuals risk of death rather than the diabetes itself. ( Mohammadi et al., 2007) Morbidity and mortality of type 2 diabetes mellitus (DM) is closely related to development of cardiovascular disease (Haffner et al.,1998). Although the manifestations and complications of diabetes mellitus have been extensively studied, the knowledge regarding the effects of diabetes on bone mineral metabolism is still limited and inconsistent. Since Albright and Reifenstein(Anwar et al.,2008)first reported the occurrence of osteoporosis in patients with poorly controlled diabetes in 1948, many authors have attempted to describe alterations of bone mineralization in diabetic subjects by using various techniques and biochemical markers(Asbun and Villareal,2006). The types of diabetes caused by other conditions or found in increased frequency with other conditions (imply an etiologie relationship) be considered a third subclass of diabetes mellitus that is the diabetes that associated with certain conditions and syndromes. This subclass has been divided according to the known or suspected etiologie relationships.The class gestational diabetes has been restricted to women in whom glucose intolerance develops or is discovered during pregnancy (National Diabetes Data Group,1979).

Saturday, January 18, 2020

Surrogates the Movie

Surrogates: â€Å"Look at yourselves, unplug from your chairs, get up, and look in the mirror what you see is how God made you we’re not meant to experience life through a machine. † This seems to be the moral of this movie in the year 2017 and takes place in Boston, MA. Bruce Willis plays an FBI detective named Tom Greer working on a very mysterious murder of a young man who is the son of a prominent inventor. A news style montage opens the movie and provides you with the fundamental basis for the movie. The viewer learns that the technology of Surrogates took 14 years to develop into the everyday application as it would be depicted in the film. The surrogate project started as a way to help the disabled experience life in a way that would not be possible and soon was taken over by military application to use these surrogates and warriors in the battlefield to preserve human life. It was the Military application that set up mass production of surrogates and this made surrogates affordable to the average consumer. Near the end of the 14 year montage it is established that 98% of the entire world has a surrogate and is using them for their everyday life so the user can remain at home in safety and never needs to risk the danger or inconvenience of leaving home. There is a group of people however that are in strict opposition to the new surrogate way of life. This group is called the Human Coalition and they live in Dread Reservations which are located within major cities all over the world. These reservations are considered surrogate free zones and are completely sovereign. This group of people does not tolerate any type of surrogate technology and preach the experience of the human condition as the truth. This seems to be a cut and dry good vs. vil movie at first and the two sides clearly established but as the movie moves along the lines become blurred and the twists are pronounced. Tom Greer and his partner are called to the scene of a seemingly normal vehicle accident late in the evening only to discover fairly quickly that this is not just an accident scene it’s a murder. While at the scene of the accident only surrogates are present and it is assumed the operators of the surrogates remain at their homes unharmed, but after furt her investigation by Tom the operators are dead in their operator chairs. Highly unusual because there haven’t been any murders in Boston for so long the Agents do not remember the last one. And this starts the investigation into the would be conspiracy that is the plot of the movie. The inventor of surrogates is a man named Dr. Lionel Canter, who was the target of the murder but instead of killing him the murder a Human Coalition member Miles Strickland killed his son who was borrowing his father’s surrogate. Tom is convinced that the Human Coalition is responsible for the murder and takes his surrogate into the sovereign territory in Boston where his surrogate is destroyed. Tom is now forced to abandon the surrogate and continue the investigation in the flesh. This is a shocking experience for him but eventually causes him to remember the human condition and experience. No matter the difficulty he experiences as a result of the sensory overload he continues the investigation. Next Tom discovers that the military had developed the weapon that would overload many surrogates with a single shot but an accidental byproduct of this weapon was that it actually killed the operator. The Military officials ordered that all if the weapons be destroyed and somehow one slipped through the cracks and was not destroyed and fell into the hands of manufacturer of the surrogates. The manufacturer VCI is proclaimed as one of the largest companies in the world and is a very powerful corporation. It was originally founded by Dr. Canter but due to differences in philosophy he was removed as President of the company but remained a beneficiary of the profits from the sale of surrogates. The excitement builds as Tom pursues the investigation and gets closer to figuring out the conspiracy. So far he has learned that a Human Coalition member murdered the son of a Dr Canter and that the target was actually Dr Canter himself but because his son used a surrogate belonging to Dr Canter the murderer thought that he was killing him but accidentally killed his son. The military in cooperation with VCI developed a weapon that would overload surrogates and shut them down in an effort to quickly win battles without casualties. A major problem was discovered with the weapon during its initial tests and it had unintentionally killed the operators of the surrogates. The weapon was ordered to be destroyed and of the 5 that were created only 4 were actually destroyed and one remained. The military did not know who had the remaining weapon but an assumption must be made that VCI had the weapon and somehow it had fallen in to the hands of the Human Coalition who were bent on the murder of Dr Canter for creating this new society that is an abomination. Tom reveals to the military that when he was in the Human Coalitions Boston reservation he knew the weapon was there and in the hands of their leader Zaire Powell. His attempt to convince the world that the surrogates are evil seems to be failing up to now. He now has the power to shut down every single surrogate all over the world but this action would result in the death of billions of people. The Military tells Tom to stand down and that they would take over the recovery of the weapon. Tom is drawn back in when his partner is killed and her surrogate is being used to gain access to the FBI surrogate control center. He discovers that Zaire Powell is actually a surrogate being controlled by Dr. Canter. This discovery takes Tom to Dr Canters home that is protected by surrogate guards that he must take down to reach Dr Canter in time. Canter had taken control of his partners surrogate and was in the headquarters of the FBI where he can access every surrogate at one time and use the weapon to download the overload virus killing every operator connected to their surrogate. When Tom reaches Dr Canter has already begun downloading the virus. Tom confronts Dr Canter and tries to convince him that killing this many people is not the way to accomplish his goal but it’s too late and in order to prevent Tom from stopping him Dr Canter takes a cyanide pill killing himself. Tom has no choice but to take over the surrogate of his partner and try to stop the virus from taking hold and killing billions of people. With the help of the programmer at the FBI surrogate control center he is able to isolate the operators from the virus. He then has to stop the isolate the surrogates themselves from the virus but in a last minute decision decides to allow the virus to shut down all the surrogates. He had saved the lives of many people but after having experiencing the human condition again decided that everyone should have that same experience again. This movie is full of twists and changes that are hard to follow. Overall, I don’t find this movie to be a great movie. The concepts could have been developed better and the twists should have been made with more information. The twists came without warning which is good for a twist but there was no information used to explain the twists in a way that would make it believable. I have learned that this movie was first a book and it is common that the themes in a book are not completely developed when it is turned in to a movie, however the concepts could have been portrayed and explained better. I did enjoy the idea of unplugging sometimes and to experience life outside of our home and disconnect from the internet and television. There are experiences our modern technology allows us to not have and in some cases this is not always a good thing. As a child I took a vacation to Yellowstone National Park and my children have not been able to experience this yet except for on the internet. They are not excited about making this trip because they believe they have experienced this park on the internet but as many adults know it is just not the same. I am concerned that as our children develop with more access to technology they will lose touch with the human experience so any movie, book, music, or other medium that express or shows the importance of the actual physical human experience is a great thing. I would recommend this movie to anyone for this purpose and I might also suggest that in order to fully understand this movie itself that it be watched two or three times. Watch it, enjoy it but most of all experience it.

Thursday, January 9, 2020

Life After The Bloody Chamber Essay Topics

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They are, in addition, a method of social criticism. Carter's writing techniques vary between a scarcity of description, including the consummation of the principal characters' marriage, and lots of description, like the imagery of the ruby choker'.

Wednesday, January 1, 2020

Market Research Plan - 3546 Words

Lodgepole Creek Certified Organic Foods Market Research Plan For Dr. Bensen Prepared by: Stephanie Disney 1 Executive Summary of research and findings Lodgepole Creek Certifies Organic Foods would like to know who they should be marketing to, in order to receive the best results for their companys success. They would also like to know how to best appeal to this marketing mix to gain their loyalty and to compete within the market. Three research objectives are identified: who should Lodgepole Creeks marketing mix consist of; research should be done to see how Lodgepole Creek can gain their loyalty. Researching and understanding what consumers want from organic sprouting companies will†¦show more content†¦Lodgepole Creek Certified Organic Foods was founded in 1999. LC is committed to a healthier way of eating, one sprout at a time. LC grows and harvests a wide variety of products at their most nutritious stage; sprouts. Lodgepole Creek does not have a lot of competition. 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Marketing plan can be product specific, market specific or companywide plan that describes the activates that is involved in achieving marketing objectives, activities such as marketing strategyRead MoreBSBMKG506B Plan Market Research Assessment11 13445 Words   |  14 Pages Student Signature: Date of Submission: / /20 Unit description This unit describes the performance outcomes, skills and knowledge required to plan market research by identifying market research needs, defining market research objectives, identifying data gathering approaches and developing a market research plan. No licensing, legislative, regulatory or certification requirements apply to this unit at the time of endorsement. Prerequisite Requirements Evidence of completionRead MoreEssay on Starbucks Market Research Implementation Plan 2130 Words   |  9 PagesMarket Research Implementation Plan Former Starbucks Executive Scott Bedbury once said â€Å"Brands need to communicate that they are along for the ride. They are made of flesh and emotion. That they are made possible by people† (10 Quotes from Starbucks Executives, 2010, para. 6). Starbucks’ beliefs in customer service, community solitude, and a strong business core stretch far behind just a belief in a quality caffeinated product. â€Å"We make sure everything we do honors that connection – from ourRead MoreMarket Research Implementation Plan: Problem Identification and Project Outline1548 Words   |  7 PagesMarket Research Implementation Plan: Problem Identification and Project Outline From humble beginnings in 1901 to current day, Harley-Davidson strives to build and provide not only a mode of transportation but also a lifestyle. According to Harley-Davidson (2001-2011), the company mission reads â€Å"We ride with our customers and apply this deep connection in every market we serve to create superior value for all of our stakeholders† (Company, para. 2). The company always has not experienced theRead MoreAssessment BSBMKG506B Plan Market Research CAROLINE CAMARGO PRATES4127 Words   |  17 Pagesï » ¿ BSBMKG506B Plan market research 2014 Course handout and assessment Student name/number Caroline Camargo Prates BUSINESS – DIPLOMA OF MARKETING 51207 Unit: BSBMKG506B Plan market research assessment 1,2,3 Student’s name (print please) CAROLINE CAMARG PRATES Due date Assessment hand in Student Signature Date 1st resit Trainers Signature Date 2nd resit Trainers Signature Overall Competency Achieved C/NYC Date Achieved Trainers Signature I ____________________ declareRead MoreCase Study : Planning Market Research1441 Words   |  6 PagesAssessment Task 2: Project – Planning Market Research Part 2: Plan Market Research Date:10/08/16 Prepared by:Nichapat Point of Contact: Manager (Supervisor) Email:f_fongsaboo@hotmail.com Executive Summary: This marketing research will provide detailed insight to the higher management whether to go for a launch for men’s clothing or not and what are the reasons of consistent sales for last 2 quarters. Research team will strive to answer below questions by gathering and analysing customer data